Monitoring neural activity and [Ca2+] with genetically encoded Ca2+ indicators.

  title={Monitoring neural activity and [Ca2+] with genetically encoded Ca2+ indicators.},
  author={Thomas A. Pologruto and Ryohei Yasuda and Karel Svoboda},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  volume={24 43},
Genetically encoded Ca2+ indicators (GECIs) based on fluorescent proteins (XFPs) and Ca2+-binding proteins [like calmodulin (CaM)] have great potential for the study of subcellular Ca2+ signaling and for monitoring activity in populations of neurons. However, interpreting GECI fluorescence in terms of neural activity and cytoplasmic-free Ca2+ concentration ([Ca2+]) is complicated by the nonlinear interactions between Ca2+ binding and GECI fluorescence. We have characterized GECIs in pyramidal… CONTINUE READING