Monitoring membrane excitability in Drosophila expressing modified shaker constructs.


The Drosophila neuromuscular junction (NMJ) ranks as one of the preeminent model systems for studying synaptic development, function, and plasticity. This protocol describes the use of the two-electrode voltage clamp (TEVC) to examine potassium (K(+)) currents mediated by voltage-gated ion channels, and gives several genetic and pharmacological methods that… (More)
DOI: 10.1101/pdb.prot067801