Monitoring ligand-mediated internalization of G protein-coupled receptor as a novel pharmacological approach.

@article{Fukunaga2006MonitoringLI,
  title={Monitoring ligand-mediated internalization of G protein-coupled receptor as a novel pharmacological approach.},
  author={Shin'ichi Fukunaga and Shingo Setoguchi and Akira Hirasawa and Gozoh Tsujimoto},
  journal={Life sciences},
  year={2006},
  volume={80 1},
  pages={17-23}
}
Agonist activation of a G protein-coupled receptor (GPCR) results in the redistribution of the receptor protein away from the cell surface into internal cellular compartments through a process of endocytosis known as internalization. Visualization of receptor internalization has become experimentally practicable by using fluorescent reagents such as green fluorescent protein (GFP). In this study, we examined whether the ligand-mediated internalization of a GPCR can be exploited for… CONTINUE READING