Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts.

@article{Delic2010MonitoringIR,
  title={Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts.},
  author={Marizela Delic and Diethard Mattanovich and Brigitte Gasser},
  journal={FEMS microbiology letters},
  year={2010},
  volume={306 1},
  pages={61-6}
}
Methods for in vivo monitoring of redox changes in different cellular compartments have been developed in recent years, and are mostly based on redox-sensitive variants of the green fluorescent protein (GFP). However, due to the thermodynamic stability of the introduced reactive disulfide bond, these sensors are limited to reducing compartments such as the cytosol and the mitochondria, and are not suited for more oxidizing environments such as the endoplasmic reticulum (ER). To overcome this… CONTINUE READING

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