Monitoring human basophil activation via CD63 monoclonal antibody 435.

@article{Knol1991MonitoringHB,
  title={Monitoring human basophil activation via CD63 monoclonal antibody 435.},
  author={E F Knol and Frederik P. J. Mul and H. M. Jansen and Jero Calafat and Dirk Roos},
  journal={The Journal of allergy and clinical immunology},
  year={1991},
  volume={88 3 Pt 1},
  pages={328-38}
}
On activation of human basophilic granulocytes with anti-IgE or with the chemotactic peptide, formyl-methionyl-leucyl-phenylalanine, the expression of the CD63 antigen on the cell surface, detected by monoclonal antibody (MAb) 435, increased up to 100-fold. The kinetics of CD63 up regulation and histamine release were identical, and a strong correlation was… CONTINUE READING