Monitoring hepatic cholesterol 7-hydroxylase activity by assay of the stable bile acid intermediate 7-hydroxy-4-cholesten-3-one in peripheral blood

@inproceedings{Glman2003MonitoringHC,
  title={Monitoring hepatic cholesterol 7-hydroxylase activity by assay of the stable bile acid intermediate 7-hydroxy-4-cholesten-3-one in peripheral blood},
  author={Cecilia G{\"a}lman and Ingela Arvidsson and B Angelin and M Rudling},
  year={2003}
}
We describe an accurate method for monitoring the enzymatic activity of hepatic cholesterol 7 -hydroxylase (C7 OH; CYP7A1), the rate-limiting and major regulatory enzyme in the synthesis of bile acids. Assay of 7 -hydroxy4-cholesten-3-one (C4), an intermediate in bile acid synthesis, revealed that the level of C4 in peripheral blood serum or plasma showed a strong correlation to the enzymatic activity of hepatic C7 OH, both at steady-state conditions ( r 0.929) as well as during the rapid… CONTINUE READING