Monitoring enzyme catalysis with mass spectrometry.


Mass spectrometry is a rapid, sensitive, and accurate quantitative approach for the direct monitoring of enzyme-catalyzed reactions that does not require a chromophore or radiolabeling and thus provides a viable alternative to existing analytical techniques. In this study the proteolysis of intact viral capsid proteins, the alpha-glucosidase-catalyzed… (More)


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@article{Bothner2000MonitoringEC, title={Monitoring enzyme catalysis with mass spectrometry.}, author={Brian P Bothner and Ramiro A. Chavez and Jiayong Wei and Christian Strupp and Quang Phung and Anette Schneemann and Gary Siuzdak}, journal={The Journal of biological chemistry}, year={2000}, volume={275 18}, pages={13455-9} }