Monitoring endoplasmic reticulum stress responsive mRNAs by RNA sequencing.


Gene expression profile upon endoplasmic reticulum (ER) stress was analyzed by deep shotgun sequencing of mRNAs (DSSR) using RNAs from polysomes or cytoplasm of the HT29 cell. Two time points, 4h after tunicamycin treatment when IRE1α signaling pathway is active and 16h after the treatment when it is inactive, were used. There was a transient decrease in… (More)
DOI: 10.1016/j.gene.2012.03.048


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