Monitoring dynamic systems with multiparameter fluorescence imaging.

  title={Monitoring dynamic systems with multiparameter fluorescence imaging.},
  author={Volodymyr Kudryavtsev and Suren Felekyan and Anna Katarzyna Wozniak and Marcelle Koenig and Carl Sandhagen and Ralf K{\"u}hnemuth and Claus A M Seidel and Filipp Oesterhelt},
  journal={Analytical and bioanalytical chemistry},
  volume={387 1},
A new general strategy based on the use of multiparameter fluorescence detection (MFD) to register and quantitatively analyse fluorescence images is introduced. Multiparameter fluorescence imaging (MFDi) uses pulsed excitation, time-correlated single-photon counting and a special pixel clock to simultaneously monitor the changes in the eight-dimensional fluorescence information (fundamental anisotropy, fluorescence lifetime, fluorescence intensity, time, excitation spectrum, fluorescence… CONTINUE READING
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