Monitoring diazepam and desmethyldiazepam concentrations in plasma by gas-liquid chromatography, with use of a nitrogen-sensitive detector.


We describe a modified [from Anal. Chem. 36, 2099 (1964)] procedure for determining diazepam and its pharmacologically active metabolite, desmethyldiazepam, in plasma, with use of a nitrogen-sensitive detector in the gas-liquid chromatography. We used medazepam as the internal reference standard. Diazepam and desmethyldiazepam are converted to their… (More)