Monitoring autophagy in yeast: the Pho8Delta60 assay.

  title={Monitoring autophagy in yeast: the Pho8Delta60 assay.},
  author={Daniel J Klionsky},
  journal={Methods in molecular biology},
Autophagy is an ubiquitous degradative process in eukaryotic cells. It is involved in various developmental programs and is also implicated in human pathophysiology. The basic process involves the formation of a cytosolic double membrane vesicle, termed an autophagosome. The autophagosome sequesters bulk cytosol, and after completion its outside membrane fuses with the limiting membrane of the lysosome/vacuole. The fusion event releases the inner single-membrane vesicle into the lysosome… CONTINUE READING