Monitoring Rhodosporidium toruloides NCYC 921 batch fermentations growing under carbon and nitrogen limitation by flow cytometry.

@article{Andrade2012MonitoringRT,
  title={Monitoring Rhodosporidium toruloides NCYC 921 batch fermentations growing under carbon and nitrogen limitation by flow cytometry.},
  author={Raoni dos Santos Andrade and Rodrigo Leal and Jos{\'e} Carlos Pereira Roseiro and Alberto Reis and Teresa Lopes da Silva},
  journal={World journal of microbiology & biotechnology},
  year={2012},
  volume={28 3},
  pages={1175-84}
}
The yeast Rhodosporidium toruloides NCYC 921 was grown on carbon or nitrogen limited batch cultures. The fermentations were monitored using traditional techniques and multi-parameter flow cytometry. The lipid content was assessed by flow cytometry in association with the fluorocrome Nile Red which emits yellow gold fluorescence when dissolved in neutral lipids and red fluorescence when dissolved in polar lipids. In this way, it was possible to at-line monitor the yeast lipid composition in… CONTINUE READING

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