Monitoring Notch activation in cultured mammalian cells: luciferase complementation imaging assays.

Abstract

Notch activation and cleavage releases the Notch intracellular domain (NICD), which translocates to the nucleus, where it associates with its DNA-binding partner CSL to recruit the coactivator MAML and additional cofactors to ultimately activate target gene expression. Taking advantage of the specific interaction between NICD and these factors, we have… (More)
DOI: 10.1007/978-1-4939-1139-4_12

Topics

Cite this paper

@article{Ilagan2014MonitoringNA, title={Monitoring Notch activation in cultured mammalian cells: luciferase complementation imaging assays.}, author={Ma Xenia G. Ilagan and Raphael Kopan}, journal={Methods in molecular biology}, year={2014}, volume={1187}, pages={155-68} }