Monitoring Neural Activity with Bioluminescence during Natural Behavior

  title={Monitoring Neural Activity with Bioluminescence during Natural Behavior},
  author={Eva A Naumann and Adam R. Kampff and David A Prober and Alexander F Schier and Florian Engert},
  booktitle={Nature Neuroscience},
Existing techniques for monitoring neural activity in awake, freely behaving vertebrates are invasive and difficult to target to genetically identified neurons. We used bioluminescence to non-invasively monitor the activity of genetically specified neurons in freely behaving zebrafish. Transgenic fish with the Ca2+-sensitive photoprotein green fluorescent protein (GFP)-Aequorin in most neurons generated large and fast bioluminescent signals that were related to neural activity… CONTINUE READING