Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor.

@article{DeLisa1999MonitoringGF,
  title={Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor.},
  author={Matthew P DeLisa and Jincai Li and Govind Rao and William A. Weigand and William E. Bentley},
  journal={Biotechnology and bioengineering},
  year={1999},
  volume={65 1},
  pages={54-64}
}
Synthesis of an operon fusion protein was investigated in batch and fed-batch cultures at high cell densities of recombinant Escherichia coli JM105 [pBAD-GFP::CAT]. Glucose-limited growth was achieved without accumulation of inhibitory byproducts allowing high cell densities (110 g L(-1) DCW) to be attained. This was believed to be the highest reported… CONTINUE READING