Monitoring Azospirillum-wheat interactions using the gfp and gusA genes constitutively expressed from a new broad-host range vector.

@article{Ramos2002MonitoringAI,
  title={Monitoring Azospirillum-wheat interactions using the gfp and gusA genes constitutively expressed from a new broad-host range vector.},
  author={Humberto J. O. Ramos and Lauren D B Roncato-Maccari and Emanuel Maltempi de Souza and Juliana R. L. Soares-Ramos and Mariangela Hungria and F{\'a}bio de Oliveira Pedrosa},
  journal={Journal of biotechnology},
  year={2002},
  volume={97 3},
  pages={243-52}
}
To monitor the colonization of wheat roots by Azospirillum brasilense, we constructed several plasmids based on the pBBR1 replicon expressing the gfp and gusA genes constitutively. Both genes were placed under control of the gentamycin resistance gene promoter resulting in high levels of expression in Escherichia coli and A. brasilense. The constructed plasmids were stably maintained in A. brasilense strains even in the absence of selective pressure. The colonization of wheat plants grown under… CONTINUE READING