Monarda fistulosa hydrolate as antimicrobial agent in artificial media for the in vitro rearing of the tachinid parasitoid Exorista larvarum

  title={Monarda fistulosa hydrolate as antimicrobial agent in artificial media for the in vitro rearing of the tachinid parasitoid Exorista larvarum},
  author={Maria Luisa Dindo and Monica Marianna Modesto and Chiara Rossi and Maura Di Vito and Giovanni Burgio and Lorenzo Barbanti and Paola Mattarelli},
  journal={Entomologia Experimentalis et Applicata},
Exorista larvarum (L.) (Diptera: Tachinidae), a larval parasitoid of Lepidoptera, can be reared from egg to fecund adult on artificial media composed of crude components. The standard in vitro culture is performed in 24‐well plastic rearing plates. Exorista larvarum eggs, removed from superparasitized larvae of Galleria mellonella (L.) (Lepidoptera: Pyralidae), are individually placed in the wells, each containing a cotton swab soaked in liquid medium. The plates are then sealed until… 

Monarda didyma Hydrolate Affects the Survival and the Behaviour of Drosophila suzukii

The insecticidal properties of hydrolate from Monarda didyma, scarlet beebalm, an aromatic herb in the Lamiaceae family is investigated, showing that thymol and carvacrol were the most abundant VOCs in the Hydrolate.

Manual inoculation of host larvae with first instar maggots as a rearing technique for the larval parasitoid Actia interrupta (Diptera: Tachinidae)

A rearing technique using manual inoculation of first instar maggots on the larval hosts is described that improves rearing efficacy and allows for laboratory experimentation.

Study of the biology of the Tachinidae family

This collection consists of bibliographical research on the Muscoid dipterans of the Tachinidae Family and conceptual aspects such as: geographical distribution, hosts, species, life cycle and reproduction.

Entomophagous insects – an introduction

This special issue contains papers presented at the 6th International Entomophagous Insects Conference and nine research articles cover the rearing, behavior, life history, and ecology of parasitoid and predator species.



In Vitro Rearing of the Parasitoid Exorista larvarum (L.) (Diptera: Tachinidae) on Meat Homogenate-Based Diets

Fly longevity and fecundity of the Females obtained on diet I were comparable to those of the females emerged from puparia formed in G. mellonella larvae, but male and female puparial weights were, however, higher and development times longer on the diet than in the host.

Supplementation of an Artificial Medium for the Parasitoid Exorista larvarum (Diptera: Tachnidae) With Hemolymph of Hermetia illucens (Diptera: Stratiomyidae) or Antheraea pernyi (Lepidoptera: Saturniidae)

The results indicate that the in vitro development of E. larvarum improved when the basic artificial medium was enriched with H. illucens hemolymph, whereas the supplementation with A. pernyi hemoly mph negatively affected the quality of theIn vitro-reared females.

In vitro rearing of the parasitoid Exorista larvarum (Diptera: Tachinidae) from eggs laid out of host.

Findings suggested that E. larvarum may be successfully reared in vitro with a total exclusion of the host insect.

Improvements in the Rearing of the Tachinid Parasitoid Exorista larvarum (Diptera: Tachinidae): Influence of Adult Food on Female Longevity and Reproduction Capacity

Pollen alone was deemed to be the most suitable food for adult females of E. larvarum, and showed a longevity-promoting effect, increasing the number of eggs laid on host larvae throughout the female lifespan.

Effects of Storage at Suboptimal Temperatures on the In Vitro-Reared Parasitoid Exorista larvarum (Diptera: Tachinidae).

It is suggested that it is possible to delay the development of the in vitro-reared E. larvarum, which eventually reached the adult stage, although some negative effects on fly quality and fecundity were also observed.

Low‐temperature storage of Exorista larvarum puparia as a tool for assisting parasitoid production

The temperature of 15 °C proved to be suitable for all the durations tested, although some detrimental effects were observed following storage (e.g., lower longevity and fecundity), and may prove useful to increase the flexibility of E. larvarum colony management.

Rearing of Exorista larvarum (Diptera Tachinidae): simplification of the in vitro technique

The tachinid Exorista larvarum (L.) was developed in vitro from egg to adult using absorbent cotton in replacement of more expensive agar as a physical support of the artificial medium. Puparial

In vitro rearing of Exorista larvarum on tissue culture‐based diets

The results demonstrate that E. larvarum is the most promising parasitoid ever studied for in vitro mass production.

Hydrosols evaluation in pest control: insecticidal and settling inhibition potential against Myzus persicae (Sulzer)

The wider use of the MWHD technique is further supported as in addition to its well‐known advantages, the effects of the hydrosols produced are comparable to those obtained by HD.

Biological and biochemical differences between in vitro‐ and in vivo‐reared Exorista larvarum

Results suggest a correlation between the amino acid deficiency and imbalance of medium‐reared larvae and the lower number of eggs laid by the females obtained, and puparial weight alone is not a reliable quality parameter for E. larvarum reared on artificial media.