Molecules participating in insect immunity of Sarcophaga peregrina

  title={Molecules participating in insect immunity of Sarcophaga peregrina},
  author={Shunji Natori},
  journal={Proceedings of the Japan Academy. Series B, Physical and Biological Sciences},
  pages={927 - 938}
  • S. Natori
  • Published 10 December 2010
  • Biology, Chemistry
  • Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
Pricking the body wall of Sarcophaga peregrina (flesh fly) larvae with a needle activated the immune system of this insect and induced various immune molecules, including antibacterial proteins, in the hemolymph. In this review, I summarize and discuss the functions of these immune molecules, with particular emphasis on the dual roles of some of these molecules in defense and development. 

131-142 Guo YD.pmd

This study reviewed the various aspects of this fly species, including morphology, bionomics, molecular analysis, medical and forensic entomology involvement, such as morphological characteristics of larva, puparia and adult, developmental rate of larvae, and the effects of heavy metal on the growth and developmentin of larvae.


This study investigated the antimicrobial activity of maggot hemolymph from Sarcophaga argyrostama, a common species of flesh flies in Egypt and showed thathemolymph was more effective against Gram positive bacteria especially Bacillus subtilis and effective against the filamentous fungus Geotrichum candidum.

Functional analysis of hemolin gene from silkworm, Bombyx mori - immune and development.

It is found that the transcription and expression of hemolin gene could be up-regulated effectively after injection of inactive Escherichia coli and cell wall components into silkworm midgut and no any changes in transcription andexpression were observed by the injection of BmNPV.

In vitro antimicrobial activity of maggot excretions/secretions of Sarcophaga (Liopygia) argyrostoma (Robineau-Desvoidy)

The antibacterial properties of S. argyrostoma ES were not affected by heating or freeze-thaw cycles when tested against E. coli, and the ES product produced by third instar maggots proved to be more effective against Gram-ve bacteria.

Lipopolysaccharide O-antigen of enterohemorrhagic Escherichia coli O157:H7 is required for killing both insects and mammals.

It is suggested that the LPS O-antigen of EHEC O157:H7 plays an important defensive role against antimicrobial factors in the host body fluid and is thus essential to the lethal effects of EhEC in animals.

Utilization of a silkworm model for understanding host-pathogen interactions

The recent utilization of silkworms as an animal infection model led to the identification of novel virulence genes of human pathogenic microorganisms and novel innate immune factors in the silkworm.

Pierisins and CARP-1: ADP-ribosylation of DNA by ARTCs in butterflies and shellfish.

The physiological role of pierisin in the butterfly is suggested to be a defense factor against parasitization by wasps, and other type of DNA ADP-ribosyltransferase is present in certain kinds of edible clams.

Identification of a novel strong promoter from the anhydrobiotic midge, Polypedilum vanderplanki, with conserved function in various insect cell lines

The identification of a new promoter, 121, which has strong constitutive transcriptional activity in Pv11 cells and promotes effective expression of exogenous genes, shows the 121 promoter to be a versatile tool for exogenous gene expression in a wide range of insect cell lines, particularly useful to those from non-model insect species.

Insect-Based Medicines: A Review of Present Status and Prospects of Entomo-Therapeutic Resources for Human Ailment

This review collates the most important works conducted on insects used for different medicine and includes a discussion of the potentialities of such medicines.

Insect natural products and processes: new treatments for human disease.



Mode of action of a bactericidal protein induced in the haemolymph of Sarcophaga peregrina (flesh-fly) larvae.

The mode of action of a bactericidal protein (sarcotoxin I) purified from the haemolymph of Sarcophaga peregrina (flesh-fly) larvae was studied, focusing attention on its effect on the function of

Purification of sarcotoxin II, antibacterial proteins of Sarcophaga peregrina (flesh fly) larvae.

Three antibacterial proteins with almost identical primary structures termed sarcotoxin IIA, IIB, and IIC were purified to homogeneity from the hemolymph of third instar larvae of Sarcophaga peregrina and radioimmunoassay showed that these proteins are induced in the Hemolymph in response to injury of the larval body wall.

Purification and characterization of an antibacterial protein from haemolymph of Sarcophaga peregrina (flesh-fly) larvae.

Three antibacterial proteins were induced when the body wall of Sarcophaga peregrina (flesh-fly) larvae was injured with a hypodermic needle and one protein was found to have bactericidal activity and to be effective at a concentration of 0.1 micrograms/ml against certain Gram-negative and Gram-positive bacteria.

Novel feature of expression of the sarcotoxin IA gene in development of Sarcophaga peregrina.

Purification of lectin induced in the hemolymph of Sarcophaga peregrina larvae on injury.

The same protein having lectin activity is apparently induced under two different physiological conditions: injury of the body wall of larvae and during pupation.