Molecular weight determinations of soluble and membrane-bound fractions of choline O-acetyltransferase in rat brain.

@article{Badamchian1985MolecularWD,
  title={Molecular weight determinations of soluble and membrane-bound fractions of choline O-acetyltransferase in rat brain.},
  author={Mahnaz Badamchian and P. Thomas Carroll},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={1985},
  volume={5 8},
  pages={
          1955-64
        }
}
Three fractions of choline O-acetyltransferase (ChAT) (EC 2.3.1.6) were solubilized from a nerve ending fraction of rat forebrain using three sequential washes of an increasingly chaotrophic nature (100 mM sodium phosphate, pH 7.4; 500 mM NaCl; 2% Triton DN-65) as previously described (Benishin, C.G., and P.T. Carroll (1983) J. Neurochem. 41: 1030-1039). The molecular weights of the soluble (NaP) and membrane-bound fractions (NaCl and 2% Triton DN-65) of ChAT, following partial purification… CONTINUE READING

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