Molecular typing of canine parvovirus variants by polymerase chain reaction and restriction enzyme analysis.

@article{Kumar2010MolecularTO,
  title={Molecular typing of canine parvovirus variants by polymerase chain reaction and restriction enzyme analysis.},
  author={Manoj Kumar and Sukdeb Nandi},
  journal={Transboundary and emerging diseases},
  year={2010},
  volume={57 6},
  pages={458-63}
}
Canine parvovirus type 2 (CPV-2) is a pathogen of dogs, which causes acute gastroenteritis and lymphopenia mostly in young pups. This paper reports the incidence of CPV-2 infection in diarrhoeic dogs with an aim to define the involvement of various variants of canine parvovirus circulating in India. CPV-2a, a variant of CPV-2 was differentiated from CPV-2b by polymerase chain reaction (PCR). The samples positive for CPV-2b were further analysed by PCR and restriction endonuclease (RE) analysis… CONTINUE READING