Molecular typing of N gene and 3′ untranslated region of IBV field isolates and vaccine strains using RT-PCR and RFLP

Abstract

Whole nucleocapsid (N) gene and 3′ untranslated region (UTR) of infectious bronchitis virus (IBV) vaccines and Iranian field isolates were amplified using reverse transcription and polymerase chain reaction (RT-PCR). The amplified fragments were subjected to digestion using two restriction endonuclease enzymes, AluI and MnlI. Five different restriction… (More)
DOI: 10.1007/s00580-010-1093-3

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@article{Majdani2010MolecularTO, title={Molecular typing of N gene and 3′ untranslated region of IBV field isolates and vaccine strains using RT-PCR and RFLP}, author={Raheleh Majdani and K. and Khalili Mardani and Ahmad Morshedi and Mehdi Vasfi Marandi and A. A. Talebi and Iraj Yazdani}, journal={Comparative Clinical Pathology}, year={2010}, volume={21}, pages={283-287} }