Molecular typing of Borrelia burgdorferi sensu lato by randomly amplified polymorphic DNA fingerprinting analysis.

@article{Wang1998MolecularTO,
  title={Molecular typing of Borrelia burgdorferi sensu lato by randomly amplified polymorphic DNA fingerprinting analysis.},
  author={Gehong Wang and Alje P. van Dam and Lodewijk Spanjaard and Jacob B. Dankert},
  journal={Journal of clinical microbiology},
  year={1998},
  volume={36 3},
  pages={768-76}
}
To study whether pathogenic clusters of Borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n = 49) with various clinical entities and from ticks (n = 83) or dogs (n = 4) from different geographic regions, by randomly amplified polymorphic DNA (RAPD) fingerprinting with four arbitrary primers. The RAPD patterns were reproducible up to the 95% similarity level as shown in duplicate experiments. In these experiments the purified DNAs… CONTINUE READING

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