Molecular typing and epidemiology of enteroviruses identified from an outbreak of aseptic meningitis in Belgium during the summer of 2000.

@article{Thoelen2003MolecularTA,
  title={Molecular typing and epidemiology of enteroviruses identified from an outbreak of aseptic meningitis in Belgium during the summer of 2000.},
  author={Inge Thoelen and Philippe Lemey and Ingrid van der Donck and Kurt Beuselinck and A Michael Lindberg and Marc van Ranst},
  journal={Journal of medical virology},
  year={2003},
  volume={70 3},
  pages={420-9}
}
Non-polio enteroviruses are the most common cause of aseptic meningitis worldwide. From May to September 2000, a major outbreak of aseptic meningitis occurred in Belgium. Cerebrospinal fluid samples (CSF) of 122 patients were found to contain enterovirus RNA using diagnostic RT-PCR that targeted a 231-bp gene fragment in the 5' noncoding region. In addition, a molecular typing method was developed based on RT-nested PCR and sequencing directly from CSF(a) 358-bp fragment in the aminoterminal… CONTINUE READING
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