Molecular taxonomy of the yeasts

  title={Molecular taxonomy of the yeasts},
  author={Cletus P. Kurtzman},
The term ‘yeast’ is often taken as a synonym for Saccharomyces cerevisiae, but the phylogenetic diversity of yeasts is illustrated by their assignment to two taxonomic classes of fungi, the ascomycetes and the basidiomycetes. Subdivision of taxa within their respective classes is usually made from comparisons of morphological and physiological features whose genetic basis is often unknown. Application of molecular comparisons to questions in yeast classification offers an unprecedented… 

Genetics of the yeast, Phaffia rhodozyma

It was shown that Phaffia is closer related to the filamentous ascomycetous fungi Thermomyces lanuginosus and Aspergillus nidulans, whereas most homology was found with the basidiomycetrous yeast Filobasidiella neoformans, and an almost entirely homologous transformation sytem was developed using plasmids carrying the dominant G418 resistance gene.

Recent Concepts in Fungal Taxonomy: A Review

Techniques such as RFLP, RAPD, rDNA analysis, SSR and ISSR are are no w gaining importance in fungal systematics and related studies, which have provided new information that has caused the biolo gical- species concept to come under criticism in favor of the phylogenetic - species concept.

Molecular taxonomy of yeasts.

  • T. Deák
  • Biology
    Acta microbiologica et immunologica Hungarica
  • 1999
Based on the molecular data obtained so far several changes have been introduced in the classification of yeasts, however, substantial restructuring of current taxonomic schemes with the consequence of numerous nomenclatural changes must await further studies.

A phylogenetic analysis of the genus Saccharomyces based on 18S rRNA gene sequences: description of Saccharomyces kunashirensis sp. nov. and Saccharomyces martiniae sp. nov.

A phylogenetic investigation of the ascomycetous yeast genus Saccharomyces was performed by using 18S rRNA gene sequence analysis, showing that the genus is phylogenetically very heterogeneous.

Origins, phylogenies and relationships in the fungal Kingdom.

  • Á. Szécsi
  • Biology
    Acta microbiologica et immunologica Hungarica
  • 1999
Fungi are reproducing sexually or asexually, the diploid phase generally short-lived, and parasitize a wide range of plants, animals, and other fungi.

Diversity in organization and the origin of gene orders in the mitochondrial DNA molecules of the genus Saccharomyces.

The genome organization, i.e., the size, presence of intergenic sequences, and gene order, as well as polymorphism within the coding regions, indicate that Saccharomyces mtDNA molecules are dynamic structures and have undergone numerous changes during their evolution.

A Phylogenetic Analysis of Saccharomyces Species by the Sequence of 18S–28S rRNA Spacer Regions

Two phylogenetic trees constructed by the neighbor‐joining method showed that all the species examined were distinguished from one another and closely related and far from the Saccharomyces sensu lato species.

Sequencing as a tool in yeast molecular taxonomy.

The literature on sequencing as a tool for yeast molecular taxonomy is reviewed. Ribosomal DNA has been preferred for sequencing over other molecules such as mitochondrial DNA, and a large database

Evolution of MAT in the Candida Species Complex: Sex, Ploidy, and Complete Sexual Cycles in C. lusitaniae, C. guilliermondii, and C. krusei

The best-studied species to date are the human pathogens Candida albicans and C. glabrata. However, neither of these species is as yet known to possess a complete meiotic sexual cycle. C. lusitaniae,



Redividing the basidiomycetes on the basis of 5S rRNA sequences

5S ribosomal RNA (rRNA) nucleotide sequences from eight species of basidiomycetes define two distinct clusters that correlate with the presence or absence of cell wall septal dolipores rather than with the traditional division of these species between the classes.

Orders and Families of Ascosporogenous Yeasts and Yeast-Like Taxa Compared from Ribosomal RNA Sequence Similarities

Assessment of genera among families and orders of ascosporogenous yeasts and yeast-like fungi indicates the taxa comprise two orders and suggests certain currently accepted families are artificial.

Phylogeny among the basidiomycetous yeasts inferred from small subunit ribosomal DNA sequence.

The molecular data support the principal chemotaxonomic and ultrastructural evidence, which indicates a very close affinity between C. capitatum and L. lari-marini, and divide the eight basidiomycetous yeasts into two groups which correlated well with both septal ultrastructure and cellular xylose.

Molecular approaches to the taxonomy of ballistosporous yeasts based on the analysis of the partial nucleotide sequences of 18S ribosomal ribonucleic acids.

The mode of vegetative reproduction such as the productivity of ballistospores, stalked conidia and budding yeast cells, does not reflect the phylogenetic relationship among basidiomycetous yeasts and is considered to be a less significant taxonomic criterion than hitherto believed.

Evolutionary relationships among pathogenic Candida species and relatives

Small subunit rRNA sequences have been determined for 10 of the most clinically important pathogenic species of the yeast genus Candida and for Hansenula polymorpha and species of several other genera appear to bear specific relationships to members of the genuscandida.

Phylogenetic relationships among species of Saccharomyces, Schizosaccharomyces, Debaryomyces and Schwanniomyces determined from partial ribosomal RNA sequences

Schizosac charomyces proved to be somewhat more divergent than Saccharomyces and Debaryomyces, but species differences appear insufficient for dividing the genus, and some of the factors influencing estimates of phylogenetic distances from rRNA sequences are discussed.

Fungal Molecular Systematics

The fungi, as thus defined, are of great importance for the following reasons: (a) They are the primary decomposers in all terrestrial ecosystems; (b) they are important symbiotic associates of vascular plants both in mutualistic and parasitic relationships.