Molecular studies of the intestinal mucosal barrier physiopathology using cocultures of epithelial and immune cells: a technical update.

@article{Kernis2000MolecularSO,
  title={Molecular studies of the intestinal mucosal barrier physiopathology using cocultures of epithelial and immune cells: a technical update.},
  author={Sophie Kern{\'e}is and Elise Caliot and Hiltrud Stubbe and Anna A Bogdanova and J. P. Kraehenbuhl and Eric Pringault},
  journal={Microbes and infection},
  year={2000},
  volume={2 9},
  pages={
          1119-24
        }
}
Peyer's patch lymphocytes cocultured with Caco-2 cells trigger the phenotypic conversion of enterocytes into cells that express morphological and functional M-cell properties. We report a technical update for setting up this model, which will enable the study of M-cell biology, the identification by biochemical approaches of molecules involved in the interaction of microorganisms with M cells, and the development of vectors that would efficiently target the mucosal immune system. 
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