Molecular scatology: the use of molecular genetic analysis to assign species, sex and individual identity to seal faeces

@article{Reed1997MolecularST,
  title={Molecular scatology: the use of molecular genetic analysis to assign species, sex and individual identity to seal faeces},
  author={J. Z. Reed and D. Tollit and P. Thompson and W. Amos},
  journal={Molecular Ecology},
  year={1997},
  volume={6}
}
Seals and commercial fisheries are potential competitors for fish and cephalopods. Research into the diet of British seal species has been based on conventional dietary analyses, but these methods often do not allow assignment of species identity to scat samples. We present a protocol for obtaining DNA from seal scat (faecal) samples which can be used in polymerase chain reactions to amplify both nuclear and mitochondrial DNA. This can provide a method of identifying the species, sex and… Expand
DNA-based identification of salmonid prey species in seal faeces
TLDR
The feasibility of using faecal DNA to detect the presence of salmonids in pinniped scat samples and to distinguish reliably between sea trout Salmo trutta and Atlantic salmon S. salar was assessed. Expand
Evaluation of fecal mtDNA analysis as a method to determine the geographic distribution of a rare lagomorph
TLDR
mtDNA analysis of fecal pellets is an efficient and reliable method to inventory and monitor lagomorph populations during the winter and can be extended, with minor modifications, to the monitoring of other herbivore species. Expand
Dietary separation of sympatric carnivores identified by molecular analysis of scats
TLDR
Analysis of diets of four sympatric carnivores in the flooding savannas of western Venezuela by analysing predator DNA and prey remains in faeces shows that puma and jaguar scats overlap in size, as do those of puma, ocelot and fox. Expand
Genotyping herbivore feces facilitating their further analyses
TLDR
The utility of a DNA extraction protocol that proved to be useful for sex determination from feces for non-invasive genotyping of red deer and European hare and leaves the genotyped feces intact for further analyses is examined. Expand
Terrestrial mammal feces: a morphometric summary and description.
  • M. Chame
  • Biology, Medicine
  • Memorias do Instituto Oswaldo Cruz
  • 2003
TLDR
This work presents the compilation list of fecal shape and measurements available in the literature published in North America, Eastern and Southern Africa, Europe, and new data from Brazil, and concludes that shape and diameters are the best characteristics for taxonomic identification. Expand
Molecular methods for differentiating grey seal (Halichoerus grypus) and harbour seal (Phoca vitulina) scat
TLDR
Molecular methods to differentiate the species’ scat are developed and provide a mechanism by which separate dietary analysis can be achieved for grey and harbour seals at mixed haulouts in New England. Expand
Remote collection of animal DNA and its applications in conservation management and understanding the population biology of rare and cryptic species
TLDR
Recent developments in molecular technology add substantially to the utility of 'non-invasive' samples, which provide a source of DNA that can be used to identify not only species but also individuals and their gender, which provides great potential to improve the accuracy of abundance estimates and determine behavioural parameters. Expand
DNA barcoding meets molecular scatology: short mtDNA sequences for standardized species assignment of carnivore noninvasive samples
TLDR
Evaluated segments of ATP6 and COI hold good potential as standard markers for accurate species‐level identification in the Carnivora, and case studies with faecal samples supported the suitability of these two focal markers for poor‐quality DNA and allowed an assessment of prey DNA co‐amplification. Expand
Genotyping faeces links individuals to their diet
TLDR
The approach allows the re-sampling of individuals over time and space, and thus may be generally useful for the testing of optimal foraging theory hypotheses in mammals and also has conservation applications. Expand
Noninvasive methodology for the sampling and extraction of DNA from free-ranging Atlantic spotted dolphins ( Stenella frontalis )
TLDR
A sampling and extraction protocol for template DNA from faecal material collected in a marine environment from small cetaceans that enables species identification as well as confirmation of genetic relatedness and should be considered as a noninvasive alternative to current protocols. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 64 REFERENCES
Microsatellite markers for the study of cetacean populations
TLDR
The isolation and characterization of 12 cetacean microsatellites are described and no relationship was found between microsatellite repeat length and proportion of species which gave polymorphic products. Expand
Worldwide patterns of mitochondrial DNA differentiation in the harbor seal (Phoca vitulina).
TLDR
The results suggest that harbor seals are regionally philopatric, on the scale of several hundred kilometers, however, genetic discontinuities may exist, even between neighboring populations such as those on the Scottish and east English coasts or the east and west Baltic. Expand
Conservation genetics of the European brown bear ‐ a study using excremental PCR of nuclear and mitochondrial sequences
TLDR
It is shown that the Brenta population is monomorphic for one mitochondrial lineage and that female as well as male bears exist in the area and the implications for the management of brown bears in the BrentA and elsewhere in Europe are discussed. Expand
Sexing free‐ranging brown bears Ursus arctos using hairs found in the field
As an aid to the management of the Pyrenean population of the brown bear Ursus arctos, a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and testedExpand
Amplification of hypervariable simple sequence repeats (microsatellites) from excremental DNA of wild living bonobos (Pan paniscus)
We show that nuclear DNA extracted from faeces of free living bonobos (Pan paniscus) can be used to amplify hypervariable simple sequence repeats, which can be used for paternity analysis and kinshipExpand
Primers for the differential amplification of the sex‐determining region Y gene in a range of mammal species
TLDR
Primers to SRY allow the amplification of a 216bp product from the SRY gene in representatives of six of the eight mammalian orders tested and are designed to complement part of the conserved HMG-box region common to members of the SOX gene family. Expand
DNA sequence from the SRY gene of the sperm whale (Physeter macrocephalus) for use in molecular sexing
We sequenced a 152 base pair fragment of the sperm whale (Physeter macrocephalus) SRY gene in order to obtain species-specific primers for determination of sex by the polymerase chain reaction (PCR).Expand
Microsatellite variation in grey seals (Halichoerus grypus) shows evidence of genetic differentiation between two British breeding colonies
TLDR
Although grey seals are known to range over very large areas outside the breeding season, site fidelity of adults and philopatry of pups for these breeding colonies must be sufficiently common to have effects, through genetic drift, at the sub‐population level. Expand
The Diet of Grey Seals Around Orkney and Other Island and Mainland Sites in North-Eastern Scotland
TLDR
Diet composition, by weight, was assessed by identifying and measuring otoliths from the faecal material and correcting for reduction in otolith size as a result of digestion using experimentally derived species-specific digestion coefficients. Expand
DNA extraction from Pleistocene bones by a silica-based purification method.
TLDR
The following method, which is a modification of a protocol published by Boom et a/. Expand
...
1
2
3
4
5
...