Molecular repertoire of Deinococcus radiodurans after 1 year of exposure outside the International Space Station within the Tanpopo mission

@article{Ott2020MolecularRO,
  title={Molecular repertoire of Deinococcus radiodurans after 1 year of exposure outside the International Space Station within the Tanpopo mission},
  author={Emanuel Ott and Yuko Kawaguchi and Denise K{\"o}lbl and Elke Rabbow and Petra Rettberg and Maximilian Mora and Christine Moissl-Eichinger and Wolfram Weckwerth and Akihiko Yamagishi and Tetyana Milojevic},
  journal={Microbiome},
  year={2020},
  volume={8}
}
Background The extraordinarily resistant bacterium Deinococcus radiodurans withstands harsh environmental conditions present in outer space. Deinococcus radiodurans was exposed for 1 year outside the International Space Station within Tanpopo orbital mission to investigate microbial survival and space travel. In addition, a ground-based simulation experiment with conditions, mirroring those from low Earth orbit, was performed. Methods We monitored Deinococcus radiodurans cells during early… 
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References

SHOWING 1-10 OF 68 REFERENCES
Proteometabolomic response of Deinococcus radiodurans exposed to UVC and vacuum conditions: Initial studies prior to the Tanpopo space mission
TLDR
The effectiveness of the integrative proteometabolomic approach as a tool in molecular analysis of microbial stress response caused by space-related factors is revealed.
Proteomic and Metabolomic Profiling of Deinococcus radiodurans Recovering After Exposure to Simulated Low Earth Orbit Vacuum Conditions
TLDR
Combined comparative analysis of alterations in the proteome and metabolome helps to identify molecular key players in the stress response of D. radiodurans, thus elucidating the mechanisms behind its extraordinary regenerative abilities and enabling this microorganism to withstand vacuum stress.
Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach
TLDR
This study aims to elucidate molecular key components in D. radiodurans, which are responsible for recognition and adaptation to simulated microgravity, and highlights the importance of certain signal transducer proteins, which showed higher abundances in cells grown under reduced gravity.
How radiation kills cells: survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress.
On the Stability of Deinoxanthin Exposed to Mars Conditions during a Long-Term Space Mission and Implications for Biomarker Detection on Other Planets
TLDR
Deinoxanthin proved to be a suitable easily detectable biomarker for the search of Earth-like organic pigment-containing life on other planets, with the only exception of the exposure to simulated solar radiation.
Exposure of phototrophs to 548 days in low Earth orbit: microbial selection pressures in outer space and on early earth
TLDR
The results obtained from samples exposed to >200 nm UV demonstrate the potential for epilithic colonization of land masses during that time, but that UV radiation on anoxic planets can act as a strong selection pressure on surface-dwelling organisms.
The Possible Interplanetary Transfer of Microbes: Assessing the Viability of Deinococcus spp. Under the ISS Environmental Conditions for Performing Exposure Experiments of Microbes in the Tanpopo Mission
TLDR
It is concluded that aggregated deinococcal cells will survive the yearlong exposure experiments of the International Space Station, and it is proposed that microbial cells can aggregate as an ark for the interplanetary transfer of microbes, and is named ‘massapanspermia’.
Alteration of Proteomes in First-Generation Cultures of Bacillus pumilus Spores Exposed to Outer Space
TLDR
Proteomic characterization of vegetative SAFR-032 cells from space-surviving spores is presented in comparison to a ground control, and it is found that proteins predicted to be involved in carbohydrate transport/metabolism and energy production/conversion had lower abundance than those of the ground control.
Physiologic Determinants of Radiation Resistance inDeinococcus radiodurans
TLDR
The results of growth studies and analysis of the complete D. radiodurans genomic sequence support the hypothesis that there are several defects in the bacterium that limit carbon, nitrogen, and DNA metabolism in nutritionally limiting radioactive environments.
Oxidative Stress Resistance in Deinococcus radiodurans
TLDR
These findings challenge the concept of DNA as the primary target of radiation toxicity while advancing protein damage, and the protection of proteins against oxidative damage, as a new paradigm of radiationoxicity and survival.
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