Molecular profiling of diatom assemblages in tropical lake sediments using taxon-specific PCR and Denaturing High-Performance Liquid Chromatography (PCR-DHPLC).

@article{Epp2011MolecularPO,
  title={Molecular profiling of diatom assemblages in tropical lake sediments using taxon-specific PCR and Denaturing High-Performance Liquid Chromatography (PCR-DHPLC).},
  author={Laura S Epp and Kathleen Rosmarie Stoof-Leichsenring and Martin H. Trauth and Ralph Tiedemann},
  journal={Molecular ecology resources},
  year={2011},
  volume={11 5},
  pages={842-53}
}
Here we present a protocol to genetically detect diatoms in sediments of the Kenyan tropical Lake Naivasha, based on taxon-specific PCR amplification of short fragments (approximately 100 bp) of the small subunit ribosomal (SSU) gene and subsequent separation of species-specific PCR products by PCR-based denaturing high-performance liquid chromatography… CONTINUE READING