Molecular processing of HDL by the liver during reverse cholesterol transport.

[35S)-labelled HDL was prepared from the chyle of rats after feeding [35S)methionine/cysteine. It was added to the perfusate of isolated rat spleens, pre-labelled with [3H)cholesterol and perfused simultaneously with a rat liver. This system allowed the complete process of reverse cholesterol transport to take place while the uptake of individual HDL… CONTINUE READING