Molecular physiology of cation-coupled Cl− cotransport: the SLC12 family

@article{Hebert2004MolecularPO,
  title={Molecular physiology of cation-coupled Cl− cotransport: the SLC12 family},
  author={Steven C. Hebert and David B. Mount and Gerardo Gamba},
  journal={Pfl{\"u}gers Archiv},
  year={2004},
  volume={447},
  pages={580-593}
}
The electroneutral cation-chloride-coupled cotransporter gene family (SLC12) was identified initially at the molecular level in fish and then in mammals. This nine-member gene family encompasses two major branches, one including two bumetanide-sensitive Na+-K+-2Cl− cotransporters and the thiazide-sensitive Na+:Cl− cotransporter. Two of the genes in this branch (SLC12A1 and SLC12A3), exhibit kidney-specific expression and function in renal salt reabsorption, whereas the third gene (SLC12A2) is… 
The thiazide-sensitive Na+-Cl- cotransporter: molecular biology, functional properties, and regulation by WNKs.
  • G. Gamba
  • Biology
    American journal of physiology. Renal physiology
  • 2009
TLDR
This work honoring the memory of Steve Hebert presents a brief review of current knowledge about salt and water homeostasis generated as a consequence of cloning the cotransporter, with particular emphasis on the molecular biology, physiological properties, human disease due to decreased or increased activity of the cOTransorter, and regulation of thecotransorter by a family of serine/threonine kinases known as WNK.
Physiological role of SLC12 family members in the kidney.
TLDR
An overview of the physiological roles of these transporters in the kidney is presented, with particular emphasis on the knowledge gained in the past few years.
The Na+:Cl– Cotransporter Is Activated and Phosphorylated at the Amino-terminal Domain upon Intracellular Chloride Depletion*
TLDR
RNCC is activated by a mechanism that involves amino-terminal domain phosphorylation, and two independent intracellular chloride-depleting strategies increased rNCC activity by 3-fold, synergistic and dose-dependent.
Molecular Mechanisms of Cation Transport by the Renal Na+-K+-Cl- Cotransporter
TLDR
Study of additional mutants in which variant residues between A and F were interchanged individually in the tm2-cs1a region identified two novel residues in NKCC2 that play a key role in cation transport.
Molecular physiology and pathophysiology of electroneutral cation-chloride cotransporters.
  • G. Gamba
  • Biology
    Physiological reviews
  • 2005
TLDR
This work is a comprehensive review of the knowledge that has evolved in this area and includes molecular biology of each gene, functional properties of identified cotransporters, structure-function relationships, and physiological and pathophysiological roles of each cotranporter.
Evidence for an apical Na–Cl cotransporter involved in ion uptake in a teleost fish
TLDR
It is deduced that NKCC1a is the seawater-type cotransporter involved in ion secretion by type-IV MRCs and that NCC is the freshwater-typecotransporter involved in ions absorption by types-II and type-II M RCs.
Differential expression of Na+-Cl- cotransporter and Na+-K+-Cl- cotransporter 2 in the distal nephrons of euryhaline and seawater pufferfishes.
  • A. Kato, Takayuki Muro, S. Hirose
  • Biology, Environmental Science
    American journal of physiology. Regulatory, integrative and comparative physiology
  • 2011
TLDR
The results suggest that NaCl reabsorption in the distal nephron of the fish kidney is mediated by NCC and NKCC2 in freshwater and by NK CC2 in seawater.
K-Cl cotransport function and its potential contribution to cardiovascular disease.
  • N. Adragna, P. Lauf
  • Biology
    Pathophysiology : the official journal of the International Society for Pathophysiology
  • 2007
The structural basis of function and regulation of neuronal cotransporters NKCC1 and KCC2
TLDR
Cryo-electron microscopy structures of the two neuronal cation-chloride cotransporters human NKCC1 and mouse KCC2 are presented, identifying their essential residues for ion transport and mechanisms by which phosphorylation regulates transport activity are proposed.
NH2-terminal heterogeneity in the KCC3 K+-Cl- cotransporter.
TLDR
There is significant NH(2)-terminal heterogeneity of K CC3, with particularly robust expression of KCC3b in the kidney, along with intrarenal localization and functional characterization in Xenopus laevis oocytes.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 161 REFERENCES
Molecular cloning, expression pattern, and chromosomal localization of the human Na-Cl thiazide-sensitive cotransporter (SLC12A3).
TLDR
The cloning and characterization of hTSC render it possible to study the involvement of this cotransport system in the pathogenesis of tubulopathies such as Gitelman syndrome, and the expression pattern of the human Na-Cl thiazide-sensitive cotranporter confirms the kidney specificity.
Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney.
TLDR
Northern blot analysis and in situ hybridization indicate that these transporters are expressed predominantly in kidney with an intrarenal distribution consistent with their recognized functional localization, establishing a new family of Na(+)-(K+)-Cl- cotransporters.
Primary structure and functional expression of a cDNA encoding the thiazide-sensitive, electroneutral sodium-chloride cotransporter.
TLDR
The pharmacological and kinetic characteristics of the cloned cotransporter are consistent with the properties of native thiazide-sensitive, electroneutral sodium-chloride cOTransporters in teleost urinary bladder and mammalian renal distal tubule epithelia.
Cloning and Characterization of KCC3 and KCC4, New Members of the Cation-Chloride Cotransporter Gene Family*
TLDR
The expression of mouse KCC3 in Xenopus laevis oocytes reveals the expected functional characteristics of a K+Cl− cotransporter: Cl−-dependent uptake of86Rb+ which is strongly activated by cell swelling and weakly sensitive to furosemide.
Ion transport and ligand binding by the Na-K-Cl cotransporter, structure-function studies.
  • P. Isenring, B. Forbush
  • Biology
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
  • 2001
Cloning and Functional Characterization of a Cation-Cl− Cotransporter-interacting Protein*
TLDR
The first putative heterologous CCC-interacting protein (CIP) known at present is cloned and characterized and may be part of a novel family of proteins that modifies the activity or kinetics of CCCs through heterodimer formation.
Molecular cloning and functional expression of the bumetanide-sensitive Na-K-Cl cotransporter.
TLDR
Human HEK-293 cells have been stably transfected with the shark cDNA and shown to express Na-K-Cl cotransport activity with the bumetanide sensitivity of the shark protein, which is functionally quiescent in the host cells and can be activated by depleting the cells of chloride.
Alternatively spliced isoform of apical Na(+)-K(+)-Cl(-) cotransporter gene encodes a furosemide-sensitive Na(+)-Cl(-)cotransporter.
TLDR
These studies indicate that the mBSC1-A4 isoform of the SLC12A1 gene encodes a hypotonically activated, cAMP- and furosemide-sensitive Na(+)-Cl(-) cotransporter in thick ascending limb cells.
The Na-K-Cl cotransporter of secretory epithelia.
TLDR
This review focuses on structure-function relationships within NKCC1 and on recent developments pertaining toNKCC1 regulation at cellular and molecular levels.
Sodium-potassium-chloride cotransport.
TLDR
Although the NKCC has been studied for approximately 20 years, it is only beginning to frame the broad outlines of the structure, function, and regulation of this ubiquitous ion transport mechanism.
...
1
2
3
4
5
...