Molecular phylogenetic, morphological, and mycotoxin data support reidentification of the Quorn mycoprotein fungus as Fusarium venenatum.

@article{ODonnell1998MolecularPM,
  title={Molecular phylogenetic, morphological, and mycotoxin data support reidentification of the Quorn mycoprotein fungus as Fusarium venenatum.},
  author={Kerry O’Donnell and Elizabeth Cigelnik and Howard H. Casper},
  journal={Fungal genetics and biology : FG \& B},
  year={1998},
  volume={23 1},
  pages={
          57-67
        }
}
Molecular phylogenetic, morphological, and mycotoxin data were obtained in order to investigate the relationships and identity of the Quorn mycoprotein fungus within Fusarium and to examine Quorn strains and commercial Quorn food products for trichothecene mycotoxins. Phylogenetic analyses of aligned DNA sequences obtained via the polymerase chain reaction from the nuclear 28S ribosomal DNA, nuclear ribosomal internal transcribed spacer region, and beta-tubulin gene exons and introns indicate… 
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References

SHOWING 1-10 OF 33 REFERENCES

Clustering of trichothecene-producing Fusarium strains determined from 28S ribosomal DNA sequences

The phylogenetic placement of the species based on rDNA correlated better with toxic secondary metabolite data rather than with the current classification system based on morphology.

Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous.

The results suggest that the ancestral ITS2 types may have arisen following an ancient interspecific hybridization or gene duplication which occurred prior to the evolutionary radiation of the Gibberella fujikuroi complex and related species of Fusarium.

Phylogenetic evidence indicates the important mycotoxigenic strains Fn-2, Fn-3, Fn-2B and Fn-M represent a new species of Fusarium

Parsimony analysis of the individual and combined data sets indicate that Fn-2, Fn-3, Fn -2B, and Fn-M represent an undescribed species of Fusarium.

Phylogenetic relationships among ascomycetous truffles and the true and false morels inferred from 18S and 28S ribosomal DNA sequence analysis

Phylogenetic relationships among asco- mycetous truffles and the true and false morels were examined by using sequences from two nuclear en- coded ribosomal DNA genes, showing strong support for a Tuberaceae-Helvellaceae clade which is a monophyletic sister group of a Morchellaceae-Dis- cinaceaeClade.

Progress towards a phylogenetic classification of Fusarium

Phylogenetic reconstruction from the molecular data indicates that Fusarium, Nectria and subgenus Dialonectria are paraphyletic as currently defined and a proposal for elimination of the paraphyly of these taxa is presented.

Phylogeny of some Fusarium species, as determined by large-subunit rRNA sequence comparison.

The phylogenetic tree computed from the number of nucleotide differences shows seven Fusarium species to be more closely related to one another than the eighth species, F. nivale, is to them.

Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

Trichothecene biosynthesis in Fusarium species: chemistry, genetics, and significance.

It is indicated that production of trichothecenes can enhance the severity of disease caused by Fusarium species on some plant hosts, and genetic evidence that several trICHothecene biosynthetic genes are organized in a gene cluster is discussed.

Aspergillus nidulans β-tubulin genes are unusually divergent

Isolation of a beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance

A beta-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced, and a point mutation has been identified in the cloned gene.