Molecular nature of RAPD markers from Haemophilus influenzae Rd genome.

  title={Molecular nature of RAPD markers from Haemophilus influenzae Rd genome.},
  author={Elena Mori and Pietro Li{\`o} and Simona Daly and G. Damiani and Brunella Perito and Renato Fani},
  journal={Research in microbiology},
  volume={150 2},
Despite the widespread application of the random amplified polymorphic DNA (RAPD) technique, there is no experimental evidence of the molecular mechanism of random amplification starting from a complex template. To investigate this mechanism, we cloned and sequenced 23 selected RAPD bands amplified from Haemophilus influenzae Rd genomic DNA using eight decamer primers different in GC content and/or nucleotide sequence. As the whole genome sequence of H. influenzae Rd has been reported, the… CONTINUE READING


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