Molecular model of the specificity pocket of the hepatitis C virus protease: implications for substrate recognition.

  title={Molecular model of the specificity pocket of the hepatitis C virus protease: implications for substrate recognition.},
  author={E Pizzi and Anna Tramontano and Licia Tomei and Nicola La Monica and Cristina Maria Failla and Mohita Sardana and T Wood and Raffaele De Francesco},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  pages={888 - 892}
We have built a model of the specificity pocket of the protease of hepatitis C virus on the basis of the known structures of trypsin-like serine proteases and of the conservation pattern of the protease sequences among various hepatitis C strains. The model allowed us to predict that the substrate of this protease should have a cysteine residue in position P1. This hypothesis was subsequently proved by N-terminal sequencing of two products of the protease. The success of this "blind" test… 
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