Molecular method to assess the diversity of Burkholderia species in environmental samples.

@article{Salles2002MolecularMT,
  title={Molecular method to assess the diversity of Burkholderia species in environmental samples.},
  author={Joana Falc{\~a}o Salles and Francisco Adriano de Souza and Jan Dirk van Elsas},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 4},
  pages={
          1595-603
        }
}
In spite of the importance of many members of the genus Burkholderia in the soil microbial community, no direct method to assess the diversity of this genus has been developed so far. The aim of this work was the development of soil DNA-based PCR-denaturing gradient gel electrophoresis (DGGE), a powerful tool for studying the diversity of microbial communities, for detection and analysis of the Burkholderia diversity in soil samples. Primers specific for the genus Burkholderia were developed… CONTINUE READING

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