Molecular mechanisms of the irreversible thermal denaturation of guinea-pig liver transglutaminase.

@article{Nury1990MolecularMO,
  title={Molecular mechanisms of the irreversible thermal denaturation of guinea-pig liver transglutaminase.},
  author={S Nury and J. C. Meunier},
  journal={The Biochemical journal},
  year={1990},
  volume={266 2},
  pages={487-90}
}
When transglutaminase is heated at temperatures above 40 degrees C, it loses its activity according to a two-step mechanism [Nury, Meunier & Mouranche (1989) Eur. J. Biochem. 180, 161-166]: N----X(TD)----D However, the nature of the molecular events responsible for the irreversible denaturation is still unknown. Investigation of the effects of dithiothreitol and 5,5'-dithiobis-2-nitrobenzoate on the kinetics of inactivation, titrations of ammonia released by deamidation and of thiol groups on… CONTINUE READING

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Methods Enzymol

  • S. Nury, J. C. Meunier, A. Mouranche
  • Eur. J. Biochem
  • 1989
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