Molecular mechanisms of DNA repair inhibition by caffeine.

  title={Molecular mechanisms of DNA repair inhibition by caffeine.},
  author={Christopher P. Selby and Aziz Sancar},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={87 9},
  • C. Selby, A. Sancar
  • Published 1 May 1990
  • Biology, Chemistry
  • Proceedings of the National Academy of Sciences of the United States of America
Caffeine potentiates the mutagenic and lethal effects of genotoxic agents. It is thought that this is due, at least in some organisms, to inhibition of DNA repair. However, direct evidence for inhibition of repair enzymes has been lacking. Using purified Escherichia coli DNA photolyase and (A)BC excinuclease, we show that the drug inhibits photoreactivation and nucleotide excision repair by two different mechanisms. Caffeine inhibits photoreactivation by interfering with the specific binding of… 

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