Molecular mechanisms controlling the rate and specificity of catechol O-methylation by human soluble catechol O-methyltransferase.

@article{Lautala2001MolecularMC,
  title={Molecular mechanisms controlling the rate and specificity of catechol O-methylation by human soluble catechol O-methyltransferase.},
  author={Pia Lautala and Ismo Ulmanen and Jyrki Taskinen},
  journal={Molecular pharmacology},
  year={2001},
  volume={59 2},
  pages={393-402}
}
Molecular mechanisms determining the turn-over rate and specificity of catechol O-methylation were studied by combining enzyme kinetic measurements, computational modeling of substrate properties and fitting ligands in a 3D model of the active site of the enzyme. Enzyme kinetic measurements were carried out for 46 compounds, including most clinically used catechol drugs, by using recombinant human soluble catechol O-methyltransferase (COMT). The most important mechanism decreasing the turnover… CONTINUE READING
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