Molecular mechanism of negative autoregulation of Escherichia coli crp gene.

@article{Hanamura1991MolecularMO,
  title={Molecular mechanism of negative autoregulation of Escherichia coli crp gene.},
  author={Asao Hanamura and Hiroji Aiba Aiba},
  journal={Nucleic acids research},
  year={1991},
  volume={19 16},
  pages={4413-9}
}
Transcription of the Escherichia coli crp gene encoding cAMP receptor protein (CRP) is negatively regulated by CRP-cAMP complex that binds to a specific site located downstream from the transcription start site. The binding of CRP-cAMP to this site activates transcription from a second divergent overlapping promoter. The mechanism of this negative autoregulation of the crp gene has been investigated by in vitro transcription, gel shift, DNase I footprinting, and exonuclease III protection… CONTINUE READING