Molecular mass determination and assay of venom hyaluronidases by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

@article{Cevallos1992MolecularMD,
  title={Molecular mass determination and assay of venom hyaluronidases by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.},
  author={Miguel A Cevallos and C Navarro-Duque and M Varela-Julia and A C Alag{\'o}n},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={1992},
  volume={30 8},
  pages={925-30}
}
We describe a procedure for molecular mass determination of hyaluronidases present in animal venoms from different families. Hyaluronidases can be revealed, following their electrophoretic separation in sodium dodecyl sulfate-polyacrylamide gel containing hyaluronic acid, by incubating the gel in Triton X-100 to remove sodium dodecyl sulfate and restore in situ enzyme activity. This method allows the detection of as little as 0.025 turbidity-reducing units after 2 hr incubation. All the… CONTINUE READING

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