Molecular karyotype of Entamoeba histolytica and Entamoeba invadens.

  title={Molecular karyotype of Entamoeba histolytica and Entamoeba invadens.},
  author={Jes{\'u}s Vald{\'e}s and F de la Cr{\'u}z Hern{\'a}ndez and Rodolfo Ocadiz and Esther Orozco},
  journal={Transactions of the Royal Society of Tropical Medicine and Hygiene},
  volume={84 4},
We report the size-fractionation of Entamoeba histolytica and E. invadens deoxyribonucleic acid (DNA) by pulsed field gradient electrophoresis. Using 3 different electrophoretic conditions, we were able to resolve 6 to 9 bands between 300 and 2000 kilobases (kb), distributed in 16-22 large and up to 31 small chromosomes for E. histolytica DNA. For E. invadens, 4 to 5 bands between 300 and over 2000 kb were resolved and discriminated in 4 large and 6 small chromosomes. A ribosomal probe from… CONTINUE READING