Molecular isolation and characterization of an expressed gene from the human Y chromosome.

@article{Zhang1992MolecularIA,
  title={Molecular isolation and characterization of an expressed gene from the human Y chromosome.},
  author={Jiadong Zhang and Teresa L. Yang-Feng and Ulrich Muller and Thuluvancheri K. Mohandas and Pieter J de Jong and Y. F. Lau},
  journal={Human molecular genetics},
  year={1992},
  volume={1 9},
  pages={717-26}
}
Using a positional cloning approach, we have isolated an expressed gene from a flow-sorted Y chromosome cosmid library. The isolation of this gene was based on the identification of the Y-231 cosmid that contains CpG rich sequences (HTF islands) in its human insert. The Y-231 cosmid was capable of detecting a 1.3 kb transcript in poly (A)+ RNA samples from human testis. Several cDNA clones were isolated from a human testis cDNA library constructed in lambda gt10. In addition, DNA-mediated gene… CONTINUE READING

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