Molecular inhibition of telomerase recruitment using designer peptides: an in silico approach

  title={Molecular inhibition of telomerase recruitment using designer peptides: an in silico approach},
  author={A. Jaiswal and P. Lakshmi},
  journal={Journal of Biomolecular Structure and Dynamics},
  pages={1442 - 1459}
Telomere holds special mechanism for solving end repair problems and maintaining genomic stability. Protection of telomeres 1 (POT1) which belongs to shelterin family is identified as a key protein that recruits telomerase by interacting with telomere repeat binding factors (TRB1-3). Since, deciphering the mechanism through which POT assembles telomerase is of great interest, computational approaches have been undertaken to understand the mechanism in a well- developed model system… Expand
3 Citations
Molecular dynamics simulation of telomeric single-stranded DNA and POT1
Telomeres, which consist of single-, double- and four-stranded DNA, shorten after each round of cell division. The repeated telomeric DNA sequence 5′-TTAGGG-3′ does not encode genetic information andExpand
Protein-protein interactions generate hidden feedback and feed-forward loops to trigger bistable switches, oscillations and biphasic dose-responses.
The analyses surprisingly reveal that simple reversible PPI motifs, when being embedded into signalling cascades, could give rise to extremely rich and complex regulatory dynamics in the absence of explicit positive and negative feedback loops. Expand
A fuzzy inference system for predicting allergenicity and allergic cross-reactivity in proteins
A novel fuzzy rule based system for assessing the protein for allergenicity that combines five different modules-Machine learning classifier (MLC), Motif search, Global similarity withallergen, FAO/WHO evaluation scheme, and global similarity with allergenic like putative non-allergenic (APN) to assess the protein allerGENicity. Expand


AtTRB1–3 Mediates Structural Changes in AtPOT1b to Hold ssDNA
An attempt has been made to elucidate the reason behind the interaction by analyzing molecular docking interaction between AtPOT1b and AtTRB1–3, which yielded potential residues, which could play essential role in structural modification of TRB1-3. Expand
Arabidopsis POT1A interacts with TERT-V(I8), an N-terminal splicing variant of telomerase
This interaction indicates possible mechanisms for telomerase regulation by alternative splicing and by POT1 proteins, suggesting a specific role for this paralogue in the Arabidopsis genome. Expand
Human Protection of Telomeres 1 (POT1) Is a Negative Regulator of Telomerase Activity In Vitro
It is shown here that POT1 negatively effects telomerase activity in vitro and that the DNA binding activity of POT 1 is required for telomer enzyme inhibition. Expand
Caenorhabditis elegans POT-1 and POT-2 Repress Telomere Maintenance Pathways
The results indicate that POT-1 and Pot-2 play independent roles in suppressing a telomerase-independent telomere maintenance pathway but may function together to repress telomersase. Expand
Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase
These findings integrate TRB proteins into the telomeric interactome of A. thaliana and classify the TRB1 protein as a component of plant telomeres, confirmed by the observation of shortening of telomereres in knockout mutants of the trb1 gene. Expand
Dyskerin Is a Component of the Arabidopsis Telomerase RNP Required for Telomere Maintenance
It is concluded that dyskerin is a conserved component of the telomerase RNP complex in higher eukaryotes that is required for maximal enzyme activity in vivo. Expand
Pot1 and cell cycle progression cooperate in telomere length regulation
Results indicate that Pot1 is required to prevent a telomere checkpoint mediated by another such protein, ATR, that is most likely triggered by the G-overhang, and reveal that cell cycle progression is an important component of telomeres length regulation. Expand
Interactions of putative telomere‐binding proteins in Arabidopsis thaliana: identification of functional TRF2 homolog in plants
The results suggest that one myb‐like protein, AtTRP1, is a likely homolog of TRF2, and that AtTRB1 has a similar role in recruiting AtPot1, the Arabidopsis homology of oligonucleotide‐binding‐fold‐containing proteins which bind G‐rich telomeric DNA. Expand
The Arabidopsis Pot1 and Pot2 Proteins Function in Telomere Length Homeostasis and Chromosome End Protection
Telomeres in transgenic plants expressing a truncated AtPot1 allele lacking the N-terminal oligonucleotide/oligosaccharide binding fold (P1ΔN) are 1 to 1.5 kb shorter than in the wild type, suggesting that At pot1 contributes to the positive regulation of telomere length control, and the genome instability associated with P2ΔC mutants implies that AtPot2 contributes to chromosome end protection. Expand
Comparative biology of telomeres: Where plants stand
This review will compare the current knowledge of telomeres in plants with other organisms, with special focus on the functional length of telomeric DNA, the search for TRF homologs, the family of POT1 proteins, and the recent discovery of members of the CST complex. Expand