Molecular imaging: into in vivo interaction of HIF-1alpha and HIF-2alpha with ARNT.

@article{Konietzny2009MolecularII,
  title={Molecular imaging: into in vivo interaction of HIF-1alpha and HIF-2alpha with ARNT.},
  author={Rebecca Konietzny and Anna K{\"o}nig and Christoph Wotzlaw and Andr{\'e} Bernadini and Utta Berchner-Pfannschmidt and Joachim Fandrey},
  journal={Annals of the New York Academy of Sciences},
  year={2009},
  volume={1177},
  pages={74-81}
}
Fluorescence resonance energy transfer (FRET) combined with confocal laser microscopy is a powerful tool to analyze protein-protein interaction in vivo. We have applied this combination to study the assembly of the hypoxia-inducible factor (HIF) complex in living cells under hypoxic conditions. In hypoxia, the basic helix-loop-helix/Period/ARNT/Single-minded (PAS) proteins HIF-1alpha and HIF-2alpha accumulate and are translocated into the nucleus. Here, HIF-1alpha and HIF-2alpha dimerize with… CONTINUE READING