Molecular identification tools for sibling species of Scedosporium and Pseudallescheria.

  title={Molecular identification tools for sibling species of Scedosporium and Pseudallescheria.},
  author={Michaela Lackner and Corn{\'e} H.W. Klaassen and Jacques F Meis and Albert H. G. Gerrits van den Ende and Gert Sybren de Hoog},
  journal={Medical mycology},
  volume={50 5},
The aim of this study was to develop molecular identification tools for currently recognized species of Pseudallescheria and Scedosporium through the use of species-specific primers and RFLP, so as to enhance rapid differentiation of clinically relevant species. The variability of species was established in a set of 681 Internal Transcribed Spacer (ITS) and 349 ß-tubulin (BT2) sequences. Amplified Fragment Length Polymorphism profile clustering matched with BT2 results, whereas ITS grouping was… CONTINUE READING
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