Molecular identification of roots from a grassland community using size differences in fluorescently labelled PCR amplicons of three cpDNA regions.

@article{Taggart2011MolecularIO,
  title={Molecular identification of roots from a grassland community using size differences in fluorescently labelled PCR amplicons of three cpDNA regions.},
  author={John M Taggart and James Francis Cahill and Gordon G McNickle and John Christopher Hall},
  journal={Molecular ecology resources},
  year={2011},
  volume={11 1},
  pages={
          185-95
        }
}
Elucidating patterns of root growth is essential for a better understanding of the functioning of plant-dominated ecosystems. To this end, reliable and inexpensive methods are required to determine species compositions of root samples containing multiple species. Previous studies use a range of PCR-based approaches, but none have examined a species pool greater than 10 or 30 when evaluating mixed and single species samples, respectively. We present a method that evaluates size differences in… CONTINUE READING
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