Molecular identification of bacteria associated with bacterial vaginosis.

  title={Molecular identification of bacteria associated with bacterial vaginosis.},
  author={David N. Fredricks and Tina L. Fiedler and Jeanne M Marrazzo},
  journal={The New England journal of medicine},
  volume={353 18},
BACKGROUND Bacterial vaginosis affects millions of women and is associated with several serious health conditions. The cause of bacterial vaginosis remains poorly understood despite numerous studies based on cultures. Bacteria in microbial communities can be identified without cultivation by characterizing their ribosomal DNA (rDNA) sequences. METHODS We identified bacteria in samples of vaginal fluid with a combination of broad-range polymerase-chain-reaction (PCR) amplification of 16S rDNA… 

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