Molecular identification of a role for tyrosine 167 in the function of the human intestinal proton- coupled dipeptide transporter (hPepT1).

@article{Yeung1998MolecularIO,
  title={Molecular identification of a role for tyrosine 167 in the function of the human intestinal proton- coupled dipeptide transporter (hPepT1).},
  author={Ava Kit Ying Yeung and Sandip K. Basu and Sharon K Wu and Chen Chung Chu and Curtis T. Okamoto and Sarah F Hamm-Alvarez and Hermann von Grafenstein and W. C. Shen and Kee Jun Kim and Michael Bruce Bolger and Ian S. Haworth and David Kong Ann and Vincent H L Lee},
  journal={Biochemical and biophysical research communications},
  year={1998},
  volume={250 1},
  pages={
          103-7
        }
}
hPepT1 is a proton-coupled peptide transporter that mediates the absorption of di- and tripeptides. Here we show that tyrosine 167 (Y167) in transmembrane domain 5 (TMD5) of this 12-transmembrane spanning protein contributes to its transport function. We identified this particular amino acid by a computer model of the arrangement of the TMDs of hPepT1 and investigated its role by site-directed mutagenesis and dipeptide uptake studies. [3H]Gly-sar uptake in cells transiently transfected with… CONTINUE READING
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