Molecular identification of a pancreatic lipase‐like gene involved in sex pheromone biosynthesis of Bombyx mori

  title={Molecular identification of a pancreatic lipase‐like gene involved in sex pheromone biosynthesis of Bombyx mori},
  author={Songdou Zhang and Xun Li and Zhu Bin and Mengfang Du and Xin-ming Yin and Shi-Heng An},
  journal={Insect Science},
Cytoplasmic lipid droplet (LD) lipolysis is regulated by pheromone biosynthesis activating neuropeptide (PBAN) in Bombyx mori. To elucidate the molecular mechanism of cytoplasm LD lipolysis, the pancreatic lipase‐like gene in B. mori pheromone glands (PGs), designated as B. mori pancreatic lipase‐like gene (BmPLLG), was identified in this study. Spatial expression analysis revealed that BmPLLG is a ubiquitous gene present in all studied tissues, such as PGs, brain, epidermis, egg, midgut… Expand
Glycerol-3-phosphate O-acyltransferase is required for PBAN-induced sex pheromone biosynthesis in Bombyx mori
Quantitative proteomics was utilized to comprehensively elucidate the expression dynamics of pheromone glands (PGs) during development and indicated that GPAT acts to regulate the biosynthesis of sex phersomone precursor, TAG, thus influencing PBAN-induced sex peromone production and subsequent mating behavior. Expand
A Novel Digestive Proteinase Lipase Member H-A in Bombyx mori Contributes to Digestive Juice Antiviral Activity against B. mori Nucleopolyhedrovirus
The results indicated that BmLHA did not have digestive function but had anti-BmNPV activity, and this work provides valuable data for the clarification of the molecular characterization BLipase member H-A and supplements research on proteins of anti- Bm NPV activity in B. mori. Expand
Exploring complex pheromone biosynthetic processes in the bumblebee male labial gland by RNA sequencing
This study shows that the Bombus terrestris LG transcriptome reflects both fatty acid and isoprenoid MP biosynthetic processes and identifies rational gene targets for future studies to disentangle the molecular basis of MP biosynthesis. Expand
Lepidoptera: Female sex pheromone biosynthesis and its hormonal regulation
This chapter will provide an up to date review of pheromone biosynthesis and its hormonal regulation in moths. Expand
A Sexy Moth Model – The Molecular Basis of Sex Pheromone Biosynthesis in the Silkmoth Bombyx mori
A perspective on the spectrum of studies that have made Butenandt’s “sexy” moth one of the principal models for sex pheromone biosynthesis is provided. Expand
Identification of Differentially Expressed Genes in the Pheromone Glands of Mated and Virgin Bombyx mori by Digital Gene Expression Profiling
RNAi-mediated knockdown of putative JH receptor gene, Methoprene tolerant 1 (Met1), in female pupa resulted in a significant decrease in sex pheromone production in mature females, suggesting the importance of JH in sexphersomone synthesis. Expand
Biotechnological potential of insect fatty acid-modifying enzymes
Research on insect pheromone-biosynthetic enzymes, which exhibit diverse enzymatic properties, has the potential to broaden the understanding of enzyme specificity determinants and contribute to engineering of enzymes with desired properties for biotechnological production of FA derivatives. Expand
Identification of a thioredoxin peroxidase gene involved in resistance to nucleopolyhedrovirus infection in Helicoverpa armigera with RNA interference.
The results indicated that HaTpx contributes to the susceptibility of H. armigera to NPV, and the results provide a theoretical basis for a novel strategy for developing new chemicals and microbial pesticides that target HaT559 gene for controlling H.Armigera. Expand
Host miRNAs are involved in hormonal regulation of HaSNPV‐triggered climbing behaviour in Helicoverpa armigera
Using transcriptome analysis, it is shown that two hormone signals, juvenile hormone (JH) and 20‐hydroxyecdysone (20E), are key components involved in HaSNPV‐induced tree‐top disease in Helicoverpa armigera larvae. Expand
Regulation of pheromone biosynthesis in moths.
  • R. Jurenka
  • Biology, Medicine
  • Current opinion in insect science
  • 2017
This review will highlight recent advances in the study of regulation of pheromone biosynthesis in moths by identifying the G-protein coupled receptor that binds PBAN and aspects of signal transduction to activate the biosynthesis pathway. Expand


Identification of Lipases Involved in PBAN Stimulated Pheromone Production in Bombyx mori Using the DGE and RNAi Approaches
Four lipases directly involved in PBAN-stimulated sex pheromone biosynthesis are identified, which improve the understanding of the lipases involved in releasing bombykol precursors from triacylglycerols (TAGs) within the cytoplasmic LDs. Expand
Identification of a diacylglycerol acyltransferase 2 gene involved in pheromone biosynthesis activating neuropeptide stimulated pheromone production in Bombyx mori.
RNAi analysis confirmed that the decrease in BmDGAT2 mRNA level caused a significant reduction in sex pheromone production, indicating that DGAT2 is a key enzyme regulating B. mori sex phersomone synthesis and release. Expand
Transcriptional analysis of sex pheromone biosynthesis signal genes in Bombyx mori
Six sex pheromone synthesis signal genes, including acyl coenzyme A (acyl‐CoA) desaturase (desat1), fatty acyl reductase (FAR), phermone biosynthesis activating neuropeptide receptor (PBANR), fatty acid transport protein (FATP) and store‐operated channel protein (Orai1A), were studied for their transcriptional regulations. Expand
Functional Characterization of the Bombyx mori Fatty Acid Transport Protein (BmFATP) within the Silkmoth Pheromone Gland*
Results suggest that BmFATP plays an essential role in bombykol biosynthesis by stimulating both LD accumulation and triacylglycerol synthesis via a process called vectorial acylation that couples the uptake of extracellular fatty acids with activation to CoA thioesters during pheromonogenesis. Expand
Chemical characterization of cytoplasmic lipid droplets in the pheromone-producing cells of the silkmoth, Bombyx mori.
The role of the cytoplasmic lipid droplets in the phersomone-producing cells is to store the pheromone precursor in the form of TGs and to provide it for pherOMone production in response to the external signal of PBAN. Expand
Expression of lipid storage droplet protein-1 may define the role of AKH as a lipid mobilizing hormone in Manduca sexta.
A link between AKH-stimulated lipolysis in the fat body and the expression of LSD1 is suggested and the deduced protein sequence showed a high degree of conservation compared to other insect Lsd1s, particularly in the central region of the protein (amino acids 211-276) in which the predicted lipid binding helices are found. Expand
Characterization of acyl-CoA-binding protein (ACBP) in the pheromone gland of the silkworm, Bombyx mori.
Present results suggest that ACBP plays a significant role in the production of sex pheromones regulated by the neurohormone, phersomone biosynthesis activating neuropeptide (PBAN). Expand
Hormone Signaling Linked to Silkmoth Sex Pheromone Biosynthesis Involves Ca2+/Calmodulin-dependent Protein Kinase II-mediated Phosphorylation of the Insect PAT Family Protein Bombyx mori Lipid Storage Droplet Protein-1 (BmLsd1)*
It is found that PBAN signaling involves phosphorylation of an insect PAT family protein named B. mori lipid storage droplet protein-1 (B mLsd1) and that BmLsd1 plays an essential role in the TAG lipolysis associated with bombykol production. Expand
Pheromone biosynthesis activating neuropeptide (PBAN): regulatory role and mode of action.
  • A. Rafaeli
  • Biology, Medicine
  • General and comparative endocrinology
  • 2009
The results raise many questions concerning the evolutionary role of the PBAN/Pyrokinin receptors belonging to the GPCR family. Expand
cDNA cloning of acyl-CoA desaturase homologs in the silkworm, Bombyx mori.
Abundance of the Delta(9) desaturase clones (eight out of the 762 randomly sequenced clones) in the library prepared from diapause-destined embryos (40h after oviposition) suggests that the Delta (9) Desaturase encoded by desat 3 plays an important role in embryonic development in B. mori. Expand