Molecular identification of a new begomovirus associated with leaf crumple disease of Jatropha curcas L. in India

  title={Molecular identification of a new begomovirus associated with leaf crumple disease of Jatropha curcas L. in India},
  author={Ashish Srivastava and Meraj Jaidi and S K Kumar and Shri Krishna Raj},
  journal={Archives of Virology},
Association of a new begomovirus with leaf crumple disease of Jatropha curcas was identified based on sequence analysis of cloned ~2.7-kb viral DNA genomes from three representative samples amplified by RCA using phi-29 DNA polymerase. Sequence analysis of these isolates SKJ1 (KM066975), SKJ2 (KM189818) and SKJ3 (KM189819)—showed the presence of a monopartite begomovirus-like genome of 2,735 to 2,738 nucleotides containing seven ORFs: V3, V2 and V1 in virion sense and C3, C2, C1 and C4 in the… 
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