Molecular genetics of met17 and met25 mutants of Saccharomyces cerevisiae: Intragenic complementation between mutations of a single structural gene

@article{DAndrea1987MolecularGO,
  title={Molecular genetics of met17 and met25 mutants of Saccharomyces cerevisiae: Intragenic complementation between mutations of a single structural gene},
  author={Richard J. D'Andrea and Yolande Surdin-Kerjan and Glenn Pure and H{\'e}l{\`e}ne Cherest},
  journal={Molecular and General Genetics MGG},
  year={1987},
  volume={207},
  pages={165-170}
}
We cloned the MET17 gene of Saccharomyces cerevisiae by functional complementation after transformation of a yeast met17 mutant. Restriction mapping and nucleotide sequencing of the MET17 clones revealed that these were from the same genomic region as clones isolated previously and shown to contain the MET25 gene encoding the enzyme O-acetylhomoserine, O… CONTINUE READING